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Assembly of a human genome from nanopore sequencing data

January 8, 2017

An international consortium recently released ~30x coverage of a human immortalized cell line (NA12878) sequenced using Oxford Nanopore MinION instruments. Release 3 of this dataset included 39 flowcells, which generated 14,183,584 reads and 91,240,120,433 bases, mostly using the 1D ligation prep, but with a few rapid kit runs as well. Our friends Nick Loman and Jared Simpson asked if we could assemble this data with Canu. Of course we said yes.

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